Monkeypox Virus (MPXV) IgG ELISA Kit

• Cat.No.: E-EL-E620

Monkeypox virus (MPV) is an orthopoxvirus that causes a disease with symptoms similar, but less severe, to smallpox. While smallpox was eradicated in 1980, monkeypox continues to occur in countries of central and west Africa. Two distinct clade are identified: the west African clade and the Congo Basin clade, also known as the central African clade. In May 2022, multiple cases of monkeypox were detected in several non-endemic countries, making it urgent to further understand the epidemiology, source of infection and mode of transmission of this disease.

Monkeypox virus (MPV) is a double-stranded DNA virus belonging to orthopoxviruses of the Poxviridae family. It is one of the human orthopoxviruses, including variola (VACV), vaccinia (CPX) and vaccinia (VACV) viruses. Monkeypox virus is about 200-400 nm in size, brick or oval in shape, with stable DNA structure, which can repair itself when mutation occurs.

Specifications

Intended use

This ELISA kit applies to the in vitro qualitative determination of Monkeypox Virus (MPXV) IgG in human serum and plasma.

Performance

1. Cut Off(C.O.) = 2.1 × Negative Control(NC) average A₄₅₀.

When NC average A₄₅₀ ＜ 0.05, calculate it as 0.05. While 0.05 ≤ NC average A₄₅₀ ≤ 0.15, calculate it as the actual value.

l  Positive result: Sample absorbance ≥ Cut Off

l  Negative result: Sample absorbance ＜ Cut Off

2. Intra-CV: CV%＜8%

 Inter-CV: CV%＜10%

Storage

The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the reagents according to the manual.