Now, there is an Easy Step ELISA Kit to solve your scientific research troubles!
One-step method EasyStep principle
The kit adopts the sandwich method principle. Coat the specific antibody in a 96-well microplate, add the standard/sample, so that the protein in the standard or the protein in the sample binds to the antibody solid-phased on the microplate, and then add the HRP-coupled antibody , after thoroughly washing the unbound HRP-conjugated antibody, add TMB substrate to develop color. TMB is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The depth of color is positively correlated with the target protein in the sample. Use a microplate reader to measure the absorbance (OD value) at a wavelength of 450 nm, and calculate the sample concentration by drawing a standard curve.
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Flow cytometry kits by Exbio
The CellCycleFlowEx Kit is intended for cell cycle analysis using flow cytometry. The kit is suitable for testing suspensions of isolated cells, such as leukocytes isolated from peripheral blood (PBMC) or cells from tissue culture.
The TregFlowEx Kit is designed for the detection of regulatory T cells (CD4+CD25+FOXP3+ cells) in human peripheral blood or human umbilical cord blood using flow cytometry.
ApoFlowEx FITC Kit
The ApoFlowEx FITC Kit is intended for differentiation of early apoptotic, necrotic and viable cells.
Monkeypox Virus (MPXV) IgG ELISA Kit
Monkeypox virus (MPV) is an orthopoxvirus that causes a disease with symptoms similar, but less severe, to smallpox. While smallpox was eradicated in 1980, monkeypox continues to occur in countries of central and west Africa. Two distinct clade are identified: the west African clade and the Congo Basin clade, also known as the central African clade. In May 2022, multiple cases of monkeypox were detected in several non-endemic countries, making it urgent to further understand the epidemiology, source of infection and mode of transmission of this disease.
Monkeypox virus (MPV) is a double-stranded DNA virus belonging to orthopoxviruses of the Poxviridae family. It is one of the human orthopoxviruses, including variola (VACV), vaccinia (CPX) and vaccinia (VACV) viruses. Monkeypox virus is about 200-400 nm in size, brick or oval in shape, with stable DNA structure, which can repair itself when mutation occurs.
This ELISA kit applies to the in vitro qualitative determination of Monkeypox Virus (MPXV) IgG in human serum and plasma.
- Cut Off(C.O.) = 2.1 × Negative Control(NC) average A450.
When NC average A450 ＜ 0.05, calculate it as 0.05. While 0.05 ≤ NC average A450 ≤ 0.15, calculate it as the actual value.
l Positive result: Sample absorbance ≥ Cut Off
l Negative result: Sample absorbance ＜ Cut Off
- Intra-CV: CV%＜8%
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the reagents according to the manual.
BlasTaq is a strategically-engineered enzyme that has a rapid extension rate (15 s por kb) and a robust performance. This enzyme shortens reaction times by up to 70%, compared to wild-type Taq:
MegaFi is a strategically-engineered enzyme with exceptional sensitivity and ability to amplify even the most difficult templates. In addition, it has a high fidelity and and ultra-low error rate (over 1,000X less than Taq):
One-step TUNEL Assay Kit
Elabscience® improves the process of the entire line of products, and offers you a highly sensitive, fast and simple apoptosis detection kit (TUNEL) at a lower price and a better product experience, which can better assist in apoptosis research on diseases related to cell function and research and development of related drugs.
One-step TUNEL assay kit ( FITC, AF488, AF594, EV450, AF647, AF555) is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, frozen section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. The test results can be directly observed through a fluorescence microscope.
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GenomeCoV19 Detection Kit
The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.
abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider.