Monkeypox virus (MPV) is an orthopoxvirus that causes a disease with symptoms similar, but less severe, to smallpox. While smallpox was eradicated in 1980, monkeypox continues to occur in countries of central and west Africa. Two distinct clade are identified: the west African clade and the Congo Basin clade, also known as the central African clade. In May 2022, multiple cases of monkeypox were detected in several non-endemic countries, making it urgent to further understand the epidemiology, source of infection and mode of transmission of this disease.
Monkeypox virus (MPV) is a double-stranded DNA virus belonging to orthopoxviruses of the Poxviridae family. It is one of the human orthopoxviruses, including variola (VACV), vaccinia (CPX) and vaccinia (VACV) viruses. Monkeypox virus is about 200-400 nm in size, brick or oval in shape, with stable DNA structure, which can repair itself when mutation occurs.
Specifications
Intended use
This ELISA kit applies to the in vitro qualitative determination of Monkeypox Virus (MPXV) IgG in human serum and plasma.
Performance
- Cut Off(C.O.) = 2.1 × Negative Control(NC) average A450.
When NC average A450 ＜ 0.05, calculate it as 0.05. While 0.05 ≤ NC average A450 ≤ 0.15, calculate it as the actual value.
l Positive result: Sample absorbance ≥ Cut Off
l Negative result: Sample absorbance ＜ Cut Off
- Intra-CV: CV%＜8%
Inter-CV: CV%＜10%
Storage
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the reagents according to the manual.
BlasTaq is a strategically-engineered enzyme that has a rapid extension rate (15 s por kb) and a robust performance. This enzyme shortens reaction times by up to 70%, compared to wild-type Taq:
MegaFi is a strategically-engineered enzyme with exceptional sensitivity and ability to amplify even the most difficult templates. In addition, it has a high fidelity and and ultra-low error rate (over 1,000X less than Taq):
One-step TUNEL Assay Kit
Elabscience® improves the process of the entire line of products, and offers you a highly sensitive, fast and simple apoptosis detection kit (TUNEL) at a lower price and a better product experience, which can better assist in apoptosis research on diseases related to cell function and research and development of related drugs.
One-step TUNEL assay kit ( FITC, AF488, AF594, EV450, AF647, AF555) is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, frozen section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. The test results can be directly observed through a fluorescence microscope.
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